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BAM Media M121: Phenol Red Carbohydrate Broth

January 2001

Bacteriological Analytical Manual

M121
Phenol Red Carbohydrate Broth

Trypticase or proteone peptone No. 310 g
NaCl5 g
Beef extract (optional)1 g
Phenol red
(7.2 ml of 0.25% phenol red solution)
0.018 g
Distilled water1 liter
Carbohydrate*

 

*Dissolve either 5 g dulcitol, 10 g lactose, or 10 g sucrose (as specified in the Salmonella test) in this basal broth.

 

Dispense 2.5 ml portions into 13 x 100 mm test tubes containing inverted 6 x 50 mm fermentation tubes. Autoclave 10 min at 118°C. Final pH, 7.4 ± 0.2.

 

Alternatively, dissolve ingredients, omitting carbohydrate, in 800 ml distilled water with heat and occasional agitation. Dispense 2.0 ml portions into 13 x 100 mm test tubes containing inverted fermentation tubes. Autoclave 15 min at 118°C and let cool. Dissolve carbohydrate in 200 ml distilled water and sterilize by passing solution through bacteria-retaining filter. Aseptically add 0.5 ml sterile filtrate to each tube of sterilized broth after cooling to less than 45°C. Shake gently to mix. Final pH, 7.4 ± 0.2.

For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.