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BAM Media M120: Peptone Sorbitol Bile Broth

January 2001

Bacteriological Analytical Manual

Peptone Sorbitol Bile Broth

Na2HPO48.23 g
NaH2PO4 · H2O1.2 g
Bile salts No. 31.5 g
NaCl5 g
Sorbitol10 g
Peptone5 g
Distilled water1 liter


Dispense 100 ml into Wheaton bottles. Autoclave 15 min at 121°C. Final pH, 7.6 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.