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BAM Media M119: Penicillin-Streptomycin Solution

January 2001

Bacteriological Analytical Manual

Penicillin-Streptomycin Solution
(antibiotic concentrate)

Penicillin G500,000 IU
Streptomycin500,000 5g
Distilled water100 ml


Dissolve antibiotics in water and sterilize by filtration. Store at 5°C.


For V. cholerae, use commercially available penicillin G-streptomycin sulfate solution. Add 5 ml solution per 500 ml medium. Store at -20°C.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.