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BAM R90: Peptone-Tween-salt diluent

January 2001

Bacteriological Analytical Manual

Peptone-Tween-salt diluent (PTS)


Peptone1.0 g
Tween 8010.0 g
Sodium chloride30.0g
Distilled water1 liter


Dissolve ingredients in distilled water, adjust pH to 7.2 and dispense into bottles of desired volume. Autoclave 15 min at 121°C.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.