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U.S. Department of Health and Human Services


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BAM Media M192: Buffered Peptone Water (BPW)

September 2005

Bacteriological Analytical Manual

Buffered Peptone Water (BPW)

Peptone10 g
Sodium chloride5 g
Disodium phosphate3.5 g
Mono-potassium Phosphate1.5 g
Distilled water1 L

Autoclave at 121°C for 15 min. Final pH 7.2 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. Updated and revised: 29-DEC-2000.