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U.S. Department of Health and Human Services

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BAM Media M26: Bromcresol Purple Broth

January 2001

Bacteriological Analytical Manual

M26
Bromcresol Purple Broth

 

Base

Peptone10 g
Beef extract3 g
NaCl5 g
Bromcresol purple0.04 g
Distilled water1 liter

 

Dispense 2.5 ml portions of base solution into 13 x 100 mm test tubes containing inverted 6 x 50 mm fermentation tubes. Autoclave 10 min at 121°C. Final pH, 7.0 ± 0.2.

 

Sterilize stock solutions of carbohydrates (50% w/v) separately by autoclaving or, preferably, by filtration (0.2 µm pore size). Add 0.278 ± 0.002 ml stock carbohydrate solution to 2.5 ml basal medium to give 5% w/v final carbohydrate concentration.

 

For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.