BAM Media M25: Brilliant Green Lactose Bile Broth
Bacteriological Analytical Manual
Brilliant Green Lactose Bile Broth
|Brilliant green||0.0133 g|
|Distilled water||1 liter|
Dissolve peptone and lactose in 500 ml distilled water. Add 20 g dehydrated oxgall dissolved in 200 ml distilled water. The pH of this solution should be 7.0-7.5. Mix and add water to make 975 ml. Adjust pH to 7.4. Add 13.3 ml 0.1% aqueous brilliant green in distilled water. Add distilled water to make 1 liter.
Dispense into fermentation tubes, making certain that fluid level covers inverted vials.
Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.1.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.