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BAM Media M25: Brilliant Green Lactose Bile Broth

January 2001

Bacteriological Analytical Manual

Brilliant Green Lactose Bile Broth

Peptone10 g
Lactose10 g
Oxgall20 g
Brilliant green0.0133 g
Distilled water1 liter

Dissolve peptone and lactose in 500 ml distilled water. Add 20 g dehydrated oxgall dissolved in 200 ml distilled water. The pH of this solution should be 7.0-7.5. Mix and add water to make 975 ml. Adjust pH to 7.4. Add 13.3 ml 0.1% aqueous brilliant green in distilled water. Add distilled water to make 1 liter.

Dispense into fermentation tubes, making certain that fluid level covers inverted vials.

Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.1.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.