BAM Media M23: Brain Heart Infusion (BHI) Agar (0.7%)

January 2001

Bacteriological Analytical Manual

Brain Heart Infusion (BHI) Agar (0.7%) (for staphylococcal enterotoxin)

Prepare a suitable quantity of brain heart infusion broth (M24). Adjust pH to 5.3 with 1 N HCl. Add agar to give 0.7% concentration. Dissolve by minimal boiling. Dispense 25 ml portions into 25 × 200 mm test tubes. Autoclave 10 min at 121°C.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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