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BAM Media M22: Blood Agar Base #2 (Difco)

January 2001

Bacteriological Analytical Manual

Blood Agar Base #2 (Difco)

Proteose peptone15 g
Liver digest2.5 g
Yeast extract5 g
NaCl5 g
Agar12 g
Distilled Water1 liter


Autoclave at 121°C for 15 min. For blood agar, reduce water to 950 ml. Add 50 ml defibrinated (whole or lysed) horse blood and FBP (4 ml to agar + blood) after autoclaving and cooling to 48°C. Final pH, 7.4 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.