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BAM Media M21: Blood Agar Base (Infusion Agar)

January 2001

Bacteriological Analytical Manual

Blood Agar Base (Infusion Agar)

Heart muscle, infusion from375 g
Thiotone10 g
NaCl5 g
Agar15 g
Distilled water1 liter


Heat gently to dissolve. Autoclave 20 min at 121°C. Final pH, 7.3 ± 0.2. Commercially available dehydrated heart infusion agars may be used.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.