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U.S. Department of Health and Human Services


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BAM Media M20a: Blood Agar Base

January 2001

Bacteriological Analytical Manual

Blood Agar Base

Infusion from beef heart500 g
Tryptose10 g
NaCl5 g
Agar15 g
Sheep blood
(sterile, defibrinated)
50 ml
Distilled water1 liter


Suspend ingredients except blood to dissolve. Autoclave 15 min at 121°C. Cool to 45-50°C. Add 50 ml blood (room temperature), and mix. Dispense into sterile petri dishes. Final pH, 6.8 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.