Bacteriological Analytical Manual
Blood Agar Base
|Infusion from beef heart||500 g|
|Distilled water||1 liter|
Suspend ingredients except blood to dissolve. Autoclave 15 min at 121°C. Cool to 45-50°C. Add 50 ml blood (room temperature), and mix. Dispense into sterile petri dishes. Final pH, 6.8 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.