Bacteriological Analytical Manual
Bismuth Sulfite Agar (Wilson and Blair)
|Polypeptone (or peptone)||10 g|
|Beef extract||5 g|
|Na2HPO4 (anhydrous)||4 g|
|FeSO4 (anhydrous)||0.3 g|
|Bismuth sulfite (indicator)||8 g|
|Brilliant green||0.025 g|
|Distilled water||1 liter|
Mix thoroughly and heat with agitation. Boil about 1 min to obtain uniform suspension. (Precipitate will not dissolve.) Cool to 45-50°C. Suspend precipitate by gentle agitation, and pour 20 ml portions into sterile 15 × 100 mm petri dishes. Let plates dry about 2 h with lids partially removed; then close plates. Final pH, 7.7 ± 0.2
DO NOT AUTOCLAVE. Prepare plates on day before streaking and store in dark. Selectivity decreases in 48h.
|CAUTION: DO NOT AUTOCLAVE|
|PREPARATION||Day before use.|
|Loss of Selectivity after 48 h|
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.