• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services


  • Print
  • Share
  • E-mail

BAM Media M16: Arginine-Glucose Slant (AGS)

January 2001

Bacteriological Analytical Manual

Arginine-Glucose Slant (AGS)

Peptone5 g
Yeast extract3 g
Tryptone10 g
NaCl20 g
Glucose1 g
L-Arginine (hydrochloride)5 g
Ferric ammonium citrate0.5 g
Sodium thiosulfate0.3 g
Bromocresol purple0.02 g
Agar13.5 g
Distilled water1 liter


Suspend ingredients in distilled water and boil to dissolve. Dispense into tubes (for 13 x 100 mm tubes use 5 ml). Autoclave 10-12 min at 121°C. After sterilization, solidify as slants. Final pH, 6.8-7.0.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.