Bacteriological Analytical Manual
Arginine-Glucose Slant (AGS)
|Yeast extract||3 g|
|L-Arginine (hydrochloride)||5 g|
|Ferric ammonium citrate||0.5 g|
|Sodium thiosulfate||0.3 g|
|Bromocresol purple||0.02 g|
|Distilled water||1 liter|
Suspend ingredients in distilled water and boil to dissolve. Dispense into tubes (for 13 × 100 mm tubes use 5 ml). Autoclave 10-12 min at 121°C. After sterilization, solidify as slants. Final pH, 6.8-7.0.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.