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BAM Media M15: Antibiotic Medium No.4 (Agar Medium B)

January 2001

Bacteriological Analytical Manual

Antibiotic Medium No. 4 (Agar Medium B)

Tryptone or trypticase6 g
Yeast extract3 g
Beef extract1.5 g
Dextrose1 g
Agar15 g
Distilled water1 liter


Autoclave 15 min at 121°C. Final pH, 6.5-6.6. Commercially available in dehydrated form as Difco Yeast Beef Agar or BBL Yeast Beef Agar.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.