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BAM Media M13: Andrade's Carbohydrate Broth and Indicator

January 2001

Bacteriological Analytical Manual

M13
Andrade's Carbohydrate Broth and Indicator

 

Base

Beef extract3 g
Peptone or gelysate10 g
NaCl10 g
Distilled water1 liter

 

Adjust pH to 7.2 ± 0.2. Autoclave 15 min at 121°C.

 


 

Andrade's indicator

Acid fuchsin *0.2 g
1 N NaOH16 ml
Distilled water84 ml

 

Allow to decolorize before use. Add 1-2 ml NaOH if necessary. Add 10 ml indicator to 1 liter base.

* Acid fuchsin is a potential carcinogen. Avoid inhalation and contact with skin.

 

Carbohydrate stock solution.

Prepare dextrose, lactose, sucrose, and mannitol in 10% solutions. Prepare dulcitol, salicin, and other carbohydrates in 5% solutions. Sterilize by filtration through 0.20 µm membrane. Dilute sugar solutions 1:10 in base with Andrade's indicator to give recommended concentration. Mix gently.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.