Bacteriological Analytical Manual
Andrade's Carbohydrate Broth and Indicator
|Beef extract||3 g|
|Peptone or gelysate||10 g|
|Distilled water||1 liter|
Adjust pH to 7.2 ± 0.2. Autoclave 15 min at 121°C.
|Acid fuchsin *||0.2 g|
|1 N NaOH||16 ml|
|Distilled water||84 ml|
Allow to decolorize before use. Add 1-2 ml NaOH if necessary. Add 10 ml indicator to 1 liter base.
* Acid fuchsin is a potential carcinogen. Avoid inhalation and contact with skin.
Carbohydrate stock solution.
Prepare dextrose, lactose, sucrose, and mannitol in 10% solutions. Prepare dulcitol, salicin, and other carbohydrates in 5% solutions. Sterilize by filtration through 0.20 µm membrane. Dilute sugar solutions 1:10 in base with Andrade's indicator to give recommended concentration. Mix gently.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.