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U.S. Department of Health and Human Services

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BAM R77: Standard Saline Citrate (SSC) Solution (20%)

January 2001

Bacteriological Analytical Manual

R77
Standard Saline Citrate (SSC) Solution (20%)

 

NaCl (reagent grade)175.3 g
Sodium citrate88.2 g

 

Dissolve in 800 ml deionized water and adjust to pH 7 with 10 N NaOH. Bring volume to 1 liter.

 

6X SSC

 

NaCl (reagent grade)52.6 g
Sodium citrate26.5 g

 

Dissolve in 800 ml deionized water and adjust to pH 7 with 10 N NaOH. Bring volume to 1 liter.

 

3X SSC

 

6X SSC500 ml
Deionized water500 ml

 

2X SSC

 

6X SSC333 ml
Deionized water667 ml

 


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.