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BAM R75: Sonicated Calf-Thymus or Salmon-Sperm DNA

January 2001

Bacteriological Analytical Manual

R75
Sonicated Calf-Thymus or Salmon-Sperm DNA

 

DNA1 g
Distilled water1 liter

 

Stir 3-4 h to dissolve. May be heated to 60°C. Sonicate until MW is about 300,000 - 500,000 daltons. Store frozen in 1 ml aliquots.

 


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.