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BAM Media M8: Alkaline Peptone Agar

January 2001

Bacteriological Analytical Manual

Alkaline Peptone Agar

Peptone10 g
NaCl20 g
Agar15 g
Distilled water1 liter


Boil to dissolve ingredients. Adjust pH so that value after sterilization is 8.5 ± 0.2. Autoclave 15 min at 121°C. Solidify agar in tubes as slants.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.