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U.S. Department of Health and Human Services


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BAM Media M7: AKI Medium

January 2001

Bacteriological Analytical Manual

AKI Medium

Peptone15 g
Yeast extract4 g
NaCl5 g
Distilled water970 ml
10% aqueous, filter-sterilized
30 ml


On day of use, dissolve peptone, yeast extract, and NaCl in distilled water. Autoclave 15 min at 121°C. Cool. Add 30 ml freshly prepared, filter-sterilized NaHCO3, and mix. Dispense aseptically into screw-capped tubes (use 15 ml for 16 x 125 mm tubes). Final pH, 7.4 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.