Bacteriological Analytical Manual
Vibrio vulnificus Agar (VVA)
|100X Dye stock solution*||10 ml|
|Distilled water||900 ml|
Adjust to pH 8.2. Boil to dissolve agar. Autoclave for 15 min at 121°C. Temper to 50°C.
*100× Dye stock solution
|Bromthymol blue||0.6 g|
|70% Ethanol||100 ml|
For consistent medium color, use dye solution rather than repeatedly weighing out dry dyes.
|Distilled water||100 ml|
Dissolve cellobiose in distilled water by heating gently. Cool and filter sterilize.
Add Solution 2 to cooled Solution 1, mix, and dispense into petri dishes. Final color, light blue.
Medium may be stored 2 weeks at refrigeration temperatures.
|Storage:||limit: 2 wks||Temperature: 4°C|