• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services


  • Print
  • Share
  • E-mail

BAM Media M190: Vibrio vulnificus Agar (VVA)

May 2004

Bacteriological Analytical Manual

Vibrio vulnificus Agar (VVA)


Solution 1

Peptone20 g
NaCl30 g
100X Dye stock solution*10 ml
Agar25 g
Distilled water900 ml


Adjust to pH 8.2. Boil to dissolve agar. Autoclave for 15 min at 121°C. Temper to 50°C.


*100X Dye stock solution

Bromthymol blue0.6 g
70% Ethanol100 ml


For consistent medium color, use dye solution rather than repeatedly weighing out dry dyes.


Solution 2

Cellobiose10 g
Distilled water100 ml


Dissolve cellobiose in distilled water by heating gently. Cool and filter sterilize.


Add Solution 2 to cooled Solution 1, mix, and dispense into petri dishes. Final color, light blue.

Medium may be stored 2 weeks at refrigeration temperatures.


Storage:limit: 2 wksTemperature: 4°C