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BAM Media M190: Vibrio vulnificus Agar (VVA)

May 2004

Bacteriological Analytical Manual

M190
Vibrio vulnificus Agar (VVA)

 

Solution 1

Peptone20 g
NaCl30 g
100X Dye stock solution*10 ml
Agar25 g
Distilled water900 ml

 

Adjust to pH 8.2. Boil to dissolve agar. Autoclave for 15 min at 121°C. Temper to 50°C.


 

*100X Dye stock solution

Bromthymol blue0.6 g
70% Ethanol100 ml

 

For consistent medium color, use dye solution rather than repeatedly weighing out dry dyes.


 

Solution 2

Cellobiose10 g
Distilled water100 ml

 

Dissolve cellobiose in distilled water by heating gently. Cool and filter sterilize.

 

Add Solution 2 to cooled Solution 1, mix, and dispense into petri dishes. Final color, light blue.

Medium may be stored 2 weeks at refrigeration temperatures.

 

Storage:limit: 2 wksTemperature: 4°C