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BAM Media M191: Vibrio vulnificus sucrose Agar (VPSA)

May 2004

Bacteriological Analytical Manual

Vibrio vulnificus sucrose Agar (VPSA)

Tryptose5.0 g
Tryptone5.0 g
Yeast extract7.0 g
Sucrose10.0 g
NaCl30.0 g
Bile salt #31.5 g
Bromthymol blue0.025 g
Agar15.0 g
Distilled water1.0 L


Boil to dissolve ingredients and cool to 50-55ºC. Adjust pH to 6.8 with 1.0 N NaOH and dispense into sterile petri dishes.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.