• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services

Food

  • Print
  • Share
  • E-mail

BAM R64: Polymyxin B Disks, 50 Units

January 2001

Bacteriological Analytical Manual

R64
Polymyxin B Disks, 50 Units

 

Polymyxin B sulfate

Distilled water, sterilized

Filter paper disk blanks, 5-6 mm or 1/4 inch, sterilized


Micropipettor, to deliver 10 µl

 

Sterilize filter paper disks in glass petri plates. Dissolve polymyxin B in sterile water to yield a final concentration of 5000 units/ml. Place 10 1 of the 5000 units/ml solution on each disk. Dry the disks. Store desiccated and protected from light in refrigerator.

 

Example: Polymyxin B is sold by activity in USP units per mg. Check reagent bottle for activity of the lot in use. For polymyxin B with antibiotic activity of 8090 USP units per mg, dissolve 0.618 mg in 1 ml sterile water.

 

Calculation:

  5000 Units/ml  = 0.618 mg/ml
8090 Units/mg

 


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.