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U.S. Department of Health and Human Services


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BAM Media M188: Universal Preenrichment Broth

January 2001

Bacteriological Analytical Manual

Universal Preenrichment Broth

Tryptone5 g
Proteose peptone5 g
KH2PO415 g
Na2HPO47 g
NaCl5 g
Dextrose0.5 g
MgSO40.25 g
Ferric ammonium citrate0.1 g
Sodium pyruvate0.2 g
Distilled water1.0 liter


Heat with gentle agitation to dissolve. Autoclave 15 min at 121°C. Final pH, 6.3 ± 0.2.


Creation date: 2000-MAR-01