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BAM Media M167: Tryptose Broth and Agar

January 2001

Bacteriological Analytical Manual

Tryptose Broth and Agar (for serology)

Difco Bacto tryptose20 g
NaCl5 g
Dextrose1 g
Agar15 g
Distilled water1 liter


Weigh ingredients, add water, and mix. Autoclave 15 min at 121°C. Swirl to disperse molten agar. Final pH, 7.2 ± 0.2.

For broth, omit agar from formulation.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.