Food

BAM Media M166: Tryptose Blood Agar Base

January 2001

Bacteriological Analytical Manual

M166
Tryptose Blood Agar Base

Tryptose 10 g
Beef extract 3 g
NaCl 5 g
Agar 15 g
Distilled water 1 liter

Suspend ingredients in distilled water, mix thoroughly, and heat with occasional agitation. Boil about 1 min. Fill 16 × 150 mm tubes 1/3 full and cap or plug to maintain aerobic conditions. Autoclave 15 min at 121°C. Before media solidify, incline tubes to obtain 4-5 cm slant and 2-3 cm butt.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Page Last Updated: 01/28/2015
Note: If you need help accessing information in different file formats, see Instructions for Downloading Viewers and Players.