• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services


  • Print
  • Share
  • E-mail

BAM Media M166: Tryptose Blood Agar Base

January 2001

Bacteriological Analytical Manual

Tryptose Blood Agar Base

Tryptose10 g
Beef extract3 g
NaCl5 g
Agar15 g
Distilled water1 liter


Suspend ingredients in distilled water, mix thoroughly, and heat with occasional agitation. Boil about 1 min. Fill 16 x 150 mm tubes 1/3 full and cap or plug to maintain aerobic conditions. Autoclave 15 min at 121°C. Before media solidify, incline tubes to obtain 4-5 cm slant and 2-3 cm butt.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.