BAM Media M165: Tryptone Yeast Extract Agar

January 2001

Bacteriological Analytical Manual

Tryptone 10 g
Yeast extract 1 g
*Carbohydrate 10 g
Bromcresol purple 0.04 g
Agar 2 g
Distilled water 1 liter

Dissolve agar with heat and gentle agitation. Adjust pH to 7.0 ± 0.2. Fill 16 × 125 mm tubes 2/3 full. Autoclave 20 min at 115°C. Before use, steam medium 10-15 min. Solidify by placing tubes in ice water.

*Glucose and mannitol are the carbohydrates used for identification of Staphylococcus aureus.

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.


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