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U.S. Department of Health and Human Services


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BAM Media M165: Tryptone Yeast Extract Agar

January 2001

Bacteriological Analytical Manual

Tryptone Yeast Extract Agar

Tryptone10 g
Yeast extract1 g
*Carbohydrate10 g
Bromcresol purple0.04 g
Agar2 g
Distilled water1 liter


Dissolve agar with heat and gentle agitation. Adjust pH to 7.0 ± 0.2. Fill 16 x 125 mm tubes 2/3 full. Autoclave 20 min at 115°C. Before use, steam medium 10-15 min. Solidify by placing tubes in ice water.


*Glucose and mannitol are the carbohydrates used for identification of Staphylococcus aureus.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.