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BAM Media M162: Tryptone Phosphate (TP) Broth

January 2001

Bacteriological Analytical Manual

Tryptone Phosphate (TP) Broth
(for enteropathogenic Escherichia coli)

Tryptone20 g
K2HPO42 g
KH2PO42 g
NaCl5 g
Polysorbate 80 (Tween 80)1.5 ml
Distilled water1 liter


Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.