Bacteriological Analytical Manual
0.01 M Phosphate-Buffered Saline (pH 7.5)
Stock solution (0.1 M)
|Na2HPO4 (anhydrous)||12.0 g|
|NaH2PO4 · H20||2.2 g|
|Distilled water||1 liter|
Dissolve ingredients in distilled water and bring volume to 1 liter.
Dilute stock solution 1+9 in double distilled water. Mix well. Adjust pH to 7.5 with 0.1 N HCl or 0.1 N NaOH if necessary. Commercially available in dehydrated form from Difco Laboratories and BBL.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.