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BAM R60: 0.01 M Phosphate-Buffered Saline (pH 7.5)

January 2001

Bacteriological Analytical Manual

0.01 M Phosphate-Buffered Saline (pH 7.5)


Stock solution (0.1 M)


Na2HPO4 (anhydrous)12.0 g
NaH2PO4 · H202.2 g
NaCl85.0 g
Distilled water1 liter


Dissolve ingredients in distilled water and bring volume to 1 liter.


Dilute stock solution 1+9 in double distilled water. Mix well. Adjust pH to 7.5 with 0.1 N HCl or 0.1 N NaOH if necessary. Commercially available in dehydrated form from Difco Laboratories and BBL.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.