BAM R60: 0.01 M Phosphate-Buffered Saline (pH 7.5)

January 2001

Bacteriological Analytical Manual

0.01 M Phosphate-Buffered Saline (pH 7.5)

Stock solution (0.1 M)

Na2HPO4 (anhydrous) 12.0 g
NaH2PO4 · H20 2.2 g
NaCl 85.0 g
Distilled water 1 liter

Dissolve ingredients in distilled water and bring volume to 1 liter.

Dilute stock solution 1+9 in double distilled water. Mix well. Adjust pH to 7.5 with 0.1 N HCl or 0.1 N NaOH if necessary. Commercially available in dehydrated form from Difco Laboratories and BBL.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Page Last Updated: 08/03/2015
Note: If you need help accessing information in different file formats, see Instructions for Downloading Viewers and Players.