BAM R59: Phosphate-Buffered Saline (PBS), pH 7.4
Bacteriological Analytical Manual
Phosphate-Buffered Saline (PBS), pH 7.4
|Na2HPO4, anhydrous||0.724 g|
|Distilled water||1 liter|
Dissolve ingredients in distilled water. Adjust pH to 7.4 (with 1 N NaOH). Autoclave 15 min at 121°C. This buffer may be used for any step in the Vibrio spp. methods when PBS is needed.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.