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BAM R59: Phosphate-Buffered Saline (PBS), pH 7.4

January 2001

Bacteriological Analytical Manual

Phosphate-Buffered Saline (PBS), pH 7.4


NaCl7.650 g
Na2HPO4, anhydrous0.724 g
KH2PO40.210 g
Distilled water1 liter


Dissolve ingredients in distilled water. Adjust pH to 7.4 (with 1 N NaOH). Autoclave 15 min at 121°C. This buffer may be used for any step in the Vibrio spp. methods when PBS is needed.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.