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BAM R58: Peroxidase Substrate Solution (ABTS)

January 2001

Bacteriological Analytical Manual

Peroxidase Substrate Solution (ABTS)


(2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
(ABTS) [substrate]


ABTS10 mg
Citric acid (0.05 M)10 ml
Hydrogen peroxide, 30%30 1


Prepare 5 min before use. One recipe is sufficient for one 96-well microwell plate.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.