• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services

Food

  • Print
  • Share
  • E-mail

BAM R57: Peroxidase Substrate for Membrane ELISA

January 2001

Bacteriological Analytical Manual

R57
Peroxidase Substrate for Membrane ELISA

 

Solution A

 

4-Chloro-1-naphthol60 mg
Methanol (cold)20 ml

 

Dissolve 4-chloro-1-naphthol in cold methanol. Store at -20°C.

 

Solution B

 

Hydrogen peroxide, 30%60 1
Tris-buffered saline (TBS)100 ml

 

Add 30% H2O2 to TBS at room temperature.

 

Immediately before use, mix ice cold Solution A with room temperature Solution B.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.