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BAM R57: Peroxidase Substrate for Membrane ELISA

January 2001

Bacteriological Analytical Manual

Peroxidase Substrate for Membrane ELISA


Solution A


4-Chloro-1-naphthol60 mg
Methanol (cold)20 ml


Dissolve 4-chloro-1-naphthol in cold methanol. Store at -20°C.


Solution B


Hydrogen peroxide, 30%60 1
Tris-buffered saline (TBS)100 ml


Add 30% H2O2 to TBS at room temperature.


Immediately before use, mix ice cold Solution A with room temperature Solution B.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.