BAM R57: Peroxidase Substrate for Membrane ELISA

January 2001

Bacteriological Analytical Manual

Peroxidase Substrate for Membrane ELISA

Solution A

4-Chloro-1-naphthol 60 mg
Methanol (cold) 20 ml

Dissolve 4-chloro-1-naphthol in cold methanol. Store at -20°C.

Solution B

Hydrogen peroxide, 30% 60 1
Tris-buffered saline (TBS) 100 ml

Add 30% H2O2 to TBS at room temperature.

Immediately before use, mix ice cold Solution A with room temperature Solution B.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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