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BAM R49: North Aniline (Oil) - Methylene Blue Stain

January 2001

Bacteriological Analytical Manual

North Aniline (Oil)-Methylene Blue Stain


Aniline oil3 ml
Ethanol (95%)10 ml
HCl (concentrated)1.5 ml
Methylene blue (saturated solution)30 ml
Distilled water to make100 ml


Mix aniline oil with ethanol. Slowly add HCl with constant agitation. Add saturated methylene blue solution, dilute to 100 ml with water, and filter.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.