• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services

Food

  • Print
  • Share
  • E-mail

BAM R49: North Aniline (Oil) - Methylene Blue Stain

January 2001

Bacteriological Analytical Manual

R49
North Aniline (Oil)-Methylene Blue Stain

 

Aniline oil3 ml
Ethanol (95%)10 ml
HCl (concentrated)1.5 ml
Methylene blue (saturated solution)30 ml
Distilled water to make100 ml

 

Mix aniline oil with ethanol. Slowly add HCl with constant agitation. Add saturated methylene blue solution, dilute to 100 ml with water, and filter.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.