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U.S. Department of Health and Human Services


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BAM Media M6: Agar Medium P

January 2001

Bacteriological Analytical Manual

Agar Medium P

Beef extract3 gNaCl0.5 g
Peptone5 gK2HPO40.25 g
Tryptone1.7 gPolysorbate 801 g
Soytone0.3 gBromcresol purple0.06 g
Dextrose5.25 gAgar15 g
Distilled waterl liter



Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Final pH, 7.8 ± 0.2.



Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.