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BAM R45: Methylene Blue Stain (Loeffler's)

January 2001

Bacteriological Analytical Manual

Methylene Blue Stain (Loeffler's)


Solution A


Methylene blue (90% dye content) 0.3 g
Ethanol (95%) 30 


Solution B


Diluted potassium hydroxide (0.01%)

100 ml


Mix solutions A and B.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.