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BAM R45: Methylene Blue Stain (Loeffler's)

January 2001

Bacteriological Analytical Manual

R45
Methylene Blue Stain (Loeffler's)

 

Solution A

 

Methylene blue (90% dye content) 0.3 g
Ethanol (95%) 30 

 

Solution B

 

Diluted potassium hydroxide (0.01%)

100 ml

 

Mix solutions A and B.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.