BAM R45: Methylene Blue Stain (Loeffler's)

January 2001

Bacteriological Analytical Manual

Methylene Blue Stain (Loeffler's)

Solution A

Methylene blue (90% dye content) 0.3 g
Ethanol (95%) 30 

Solution B

Diluted potassium hydroxide (0.01%)

100 ml

Mix solutions A and B.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Page Last Updated: 01/29/2015
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