Bacteriological Analytical Manual
(Matheson Coleman & Bell, Norwood, OH 45212)
|Methanol (absolute)||1 liter|
Weigh stain into 50 ml methanol, dissolve by grinding, and dilute to 1 liter with methanol. Stir 16 h at 37°C. Hold stain 1 month at 22°C (room temperature). Filter before use.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.