BAM Media M4: Acid Broth

January 2001

Bacteriological Analytical Manual

Acid Broth

Proteose peptone 5 g
Yeast extract 5 g
Dextrose 5 g
K2HPO4 4 g
Distilled water l liter

Dissolve ingredients and dispense 12-15 ml portions into 20 × 150 mm tubes. Autoclave 15 min at 121°C. Final pH, 5.0.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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