• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services


  • Print
  • Share
  • E-mail

BAM Media M4: Acid Broth

January 2001

Bacteriological Analytical Manual

Acid Broth

Proteose peptone 5 g
Yeast extract 5 g
Dextrose 5 g
K2HPO4 4 g
Distilled water l liter


Dissolve ingredients and dispense 12-15 ml portions into 20 x 150 mm tubes. Autoclave 15 min at 121°C. Final pH, 5.0.



Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.