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BAM R35: Hybridization Mixture

January 2001

Bacteriological Analytical Manual

Hybridization Mixture


(6X SSC, 5X Denhardt's, 1 mM EDTA, pH 8.0)


20X SSC 15.0 ml
50X Denhardt's solution 5.0 ml
0.5 M EDTA 0.1 ml
Distilled water 28.9 ml


For hybridization with 10X Denhardt's, add 10 ml 50X Denhardt's, and decrease water volume to 23.9 ml.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.