BAM R35: Hybridization Mixture
Bacteriological Analytical Manual
(6× SSC, 5× Denhardt's, 1 mM EDTA, pH 8.0)
|20× SSC||15.0 ml|
|50× Denhardt's solution||5.0 ml|
|0.5 M EDTA||0.1 ml|
|Distilled water||28.9 ml|
For hybridization with 10X Denhardt's, add 10 ml 50X Denhardt's, and decrease water volume to 23.9 ml.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.