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BAM R34: Horseradish Peroxidase

January 2001

Bacteriological Analytical Manual

R34
Horseradish Peroxidase

 

 

HRP color development reagent 60 mg
Ice cold methanol 20 ml

 

Mix to dissolve. Protect from light and prepare fresh.

 

Solution B

 

Ice cold hydrogen peroxide, 30% 60 1
Tris-buffered saline 100 ml

 

Prepare fresh before use. Mix ice cold solution A with room temperature solution B. Use immediately.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.