BAM R34: Horseradish Peroxidase

January 2001

Bacteriological Analytical Manual

Horseradish Peroxidase

HRP color development reagent 60 mg
Ice cold methanol 20 ml

Mix to dissolve. Protect from light and prepare fresh.

Solution B

Ice cold hydrogen peroxide, 30% 60 ml
Tris-buffered saline 100 ml

Prepare fresh before use. Mix ice cold solution A with room temperature solution B. Use immediately.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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