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U.S. Department of Health and Human Services


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BAM R34: Horseradish Peroxidase

January 2001

Bacteriological Analytical Manual

Horseradish Peroxidase



HRP color development reagent 60 mg
Ice cold methanol 20 ml


Mix to dissolve. Protect from light and prepare fresh.


Solution B


Ice cold hydrogen peroxide, 30% 60 1
Tris-buffered saline 100 ml


Prepare fresh before use. Mix ice cold solution A with room temperature solution B. Use immediately.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.