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BAM R31: Glycerin-Salt Solution (Buffered)

January 2001

Bacteriological Analytical Manual

Glycerin-Salt Solution (Buffered)


Glycerin (reagent grade) 100 ml
K2HPO4 (anhydrous) 12.4 g
KH2PO4 (anhydrous) 4 g
NaCl 4.2 g
Distilled water 900 ml


Distilled NaCl and bring volume to 900 ml with water. Add glycerin and phosphates. Adjust pH to 7.2. Autoclave 15 min at 121°C. For double strength (20%) glycerin solution, use 200 ml glycerin and 800 ml distilled water.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.