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U.S. Department of Health and Human Services


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BAM R30: Giemsa Stain

January 2001

Bacteriological Analytical Manual

Giemsa Stain


Giemsa stain
(Matheson Coleman & Bell, Norwood, OH 45212)
1 g
Glycerol66 ml
Methanol (absolute)66 ml


Distilled stain in glycerol by heating 1.5-2.0 h at 55-60°C. Add methanol. Store stain in tightly stoppered bottle at 22°C for at least 2 weeks. Dilute stock solution with distilled water (1+9) before use.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.