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BAM R27: Formalinized Physiological Saline Solution

January 2001

Bacteriological Analytical Manual

Formalinized Physiological Saline Solution


Formaldehyde solution (36-38%)6 ml
NaCl8.5 ml
Distilled water1 liter


Dissolve 8.5 g NaCl in 1 liter distilled water. Autoclave 15 min at 1210C. Cool to room temperature. Add 6 ml formaldehyde solution. Do not autoclave after addition of formaldehyde.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.