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BAM R32a: Endospore Stain

January 2001

Bacteriological Analytical Manual

Endospore Stain (Schaeffer-Fulton)


Solution A

Malachite green10 g
Distilled water100 ml


Filter to remove undissolved dye.


Solution B

Safranin O0.25 g
Distilled water20 ml


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.