BAM R32a: Endospore Stain

January 2001

Bacteriological Analytical Manual

Endospore Stain (Schaeffer-Fulton)

Solution A

Malachite green 10 g
Distilled water 100 ml

Filter to remove undissolved dye.

Solution B

Safranin O 0.25 g
Distilled water 20 ml

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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