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BAM Media M2: Acetamide Medium

January 2001

Bacteriological Analytical Manual

Acetamide Medium


Stock basal medium

KH2PO40.5 M14 ml
K2HPO40.5 M6 ml
Agar0.5 g
Distilled water400 ml


Heat with agitation to dissolve agar. Add 1 ml PR-CV (500X concentrate).


Stock acetamide, 1%

Acetamide1 g
Distilled water100 ml

Store over CHCl3 in screw-cap container. Stable indefinitely at room temperature.


PR-CV (500X concentrate)

Phenol red2 g
Crystal violet0.2 g
Distilled water200 ml


Add 5 N NaOH until ingredients are dissolved.


Final medium

Add 0.8 ml basal medium to 13 x 100 mm tube. Add 0.2 ml acetamide solution. Steam (100°C) 10 min. Cool.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.