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BAM R22: ELISA Buffer

January 2001

Bacteriological Analytical Manual

ELISA Buffer


Bovine serum albumin1.0 g
NaCl8.0 g
KH2PO40.2 g
Na2HPO42.9 g
KCl0.2 g
Distilled water1 liter
Tween 200.5 ml


Adjust pH to 7.4 and add Tween 20. Store frozen. Thaw before use.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.