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BAM R19: Dulbecco's Phosphate-Buffered Saline (DPBS)

January 2001

Bacteriological Analytical Manual

Dulbecco's Phosphate-Buffered Saline (DPBS)


NaCl8.0 g
KCl200 mg
Na2HPO41.15 g
KH2PO4200 mg
CaCl2100 mg
MgCl2· 6H2O100 mg
Distilled Water1 liter


Dissolve ingredients in water. Sterilize by filtration. Final pH, 7.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.