• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services

Food

  • Print
  • Share
  • E-mail

BAM R19: Dulbecco's Phosphate-Buffered Saline (DPBS)

January 2001

Bacteriological Analytical Manual

R19
Dulbecco's Phosphate-Buffered Saline (DPBS)

 

NaCl8.0 g
KCl200 mg
Na2HPO41.15 g
KH2PO4200 mg
CaCl2100 mg
MgCl2· 6H2O100 mg
Distilled Water1 liter

 

Dissolve ingredients in water. Sterilize by filtration. Final pH, 7.2.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.