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BAM R87: Trypsin-EDTA Solution, 1X

January 2001

Bacteriological Analytical Manual

Trypsin-EDTA Solution, 1X


Trypsin (1:250)*0.05 g
EDTA, disodium salt0.02 g
NaCl, 0.9%100 ml


*1 g Trypsin (1:250) will digest 250 g casein substrate under standard conditions.


Dissolve trypsin and EDTA in the NaCl diluent. Filter-sterilize through 0.22 µm membrane.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.