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BAM R12: Catalase Test

January 2001

Bacteriological Analytical Manual

Catalase Test


Pour 1 ml 3% hydrogen peroxide over growth on slant culture. Gas bubbles indicate positive test. Alternatively, emulsify colony in l drop 3% hydrogen peroxide on glass slide. Immediate bubbling is positive catalase test. If colony is taken from blood agar plate, any carry-over of red blood cells can give false-positive reaction.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998