Bacteriological Analytical Manual
Tris-Buffered Saline (TABS), 1% or 3% Gelatin, or Tween 20
|Distilled water||1 liter|
Dissolve ingredients in distilled water by heating and stirring. Adjust pH to 7.5 with HCl. Autoclave 15 min at 121°C.
For 1% and 3% Gelatin-TABS, add 10 g and 30 g gelatin, respectively, to ingredients before autoclaving. Adjust final pH to 7.5 with HCl.
For Tween-TABS, add 0.5 ml Tween 20 to ingredient and adjust pH to 7.5 before autoclaving.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.