• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services


  • Print
  • Share
  • E-mail

BAM R81: Tris-Buffered Saline (TBS) (pH 7.5)

January 2001

Bacteriological Analytical Manual

Tris-Buffered Saline (TBS) (pH 7.5)


Tris2.42 g
NaCl29.24 g
Double distilled water to make 1 liter


Dissolve ingredients. Adjust pH to 7.5 with HCl and bring volume to 1 liter.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.